2AA Glycan Labeling Kit 2PB reductant |

2AA Glycan Labeling Kit 2PB reductant |

Application For labeling of free glycans with 2-aminobenzoic acid acid (2-AA).
Description The kit contains reagents for the conjugation of dye to the freereducing end of the glycan
by a reductive amination reaction.
Dye Properties Mass = 137. Fluorescence, ex (glycan-dye conjugate) =250 nm, em = 425 nm. For
maximum sensitivity of detection we recommend an excitation wavelengthof 250 nm.
Number of Samples 12 separate analytical samples per set of labeling reagents (24 samplesin total per kit)
Amount of Sample From 25 pmol up to 25 nmol glycans per sample.
Suitable Samples Any purified glycans with free reducing termini can be labeled.
Structural Integrity No detectable (< 2 mole per cent) loss ofsialic acid, fucose, sulfate, or phosphate.
Labeling Selectivity Essentially stoichiometric labeling.
Storage: Store at room temperature in the dark. Protect from sources of heat,light, and
moisture. The reagents are stable for at least two years as supplied.
Shipping: The product can be shipped at ambient temperature.
Handling: Ensure that any glass, plasticware or solvents used are free ofglycosidases and
environmental carbohydrates. Use powder-free gloves for all sample handling
procedures and avoid contamination with environmental carbohydrate.
All steps involving labeling reagents must be performed in a dryenvironment with dry
glassware and plasticware. Once individual vials of reagents areopened, their contents
should be used immediately and excess then discarded according to localsafety rules.
Safety: For research use only. Not for human or drug use
Please read the Material Safety Data Sheets (MSDS's) for all chemicalsused. All
processes involving labeling reagents should be performed usingappropriate personal
safety protection - eyeglasses, chemically resistant gloves (e.g.nitrile), etc. - and where
appropriate in a laboratory fume cupboard.
Kit Contents
Each kit contains two labelling reaction sets. Each labeling reactionset consists of one vial of each of the
following:
Cat. # Item Quantity
LT-2AA-03 2-AA Dye (2-aminobenzoic acid) 7.5 mg
LT-PB-01 2PB reductant (2-picoline borane) 16.5 mg
LT-ACETIC-DMSO-01 30% acetic acid in DMSO 500 μl